| ^99mTc直接标记单克隆抗体3H11及其在小鼠体内分布的研究 |
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| 引用本文： | 金建南,徐炳勋.^99mTc直接标记单克隆抗体3H11及其在小鼠体内分布的研究[J].四川大学学报(自然科学版),1997,34(5):643-647. |
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| 作者姓名： | 金建南 徐炳勋 |
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| 作者单位： | [1]四川大学原子核科学技术研究所辐射物理及技术国家教委 [2]成都416医院核医学研究所 |
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| 摘 要： | 对二硫苏糖醇（ＤＴＴ）为抗体还原剂，亚锡ＭＤＰ药盒为弱交换配体，进行了＾９９ｍＴｃ直接标记ＭｃＡｂ３Ｈ１１的研究。用ＨＰＬＣ分析鉴定标记物，标记率大于９５％，标记后不需纯化。体外竞争实验和在小鼠体内分布的研究表明，由标记得到的＾９９ｍＴｃ－３Ｈ１１和＾９９ｍＴｃ－ＩｇＧ，在体内和体外均是稳定的。
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| 关 键 词： | 单克隆抗体 直接标记法 DTT 癌 放免显像诊断 |
THE DIRECT LABELLING OF MONOCLONAL ANTIBODY 3H11 WITH 99m Tc AND BIODISTRIBUTION IN MICE |
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| Jin,Jianna,Liu,Ning,Mo,Shangwu,Cheng,Hengliu,Yu,Yanping.THE DIRECT LABELLING OF MONOCLONAL ANTIBODY 3H11 WITH 99m Tc AND BIODISTRIBUTION IN MICE[J].Journal of Sichuan University (Natural Science Edition),1997,34(5):643-647. |
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| Authors: | Jin Jianna Liu Ning Mo Shangwu Cheng Hengliu Yu Yanping |
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| Abstract: | The direct labelling of McAb3H11 with 99m Tc using dithiothreitol (DTT) as reducing agent of antibody and stannous MDP kit as weak exchange ligand was investigated.Labelled 3H11 was analysed and identified by HPLC.The labelling efficiency was more than 95%.Hence no purification was needed.Competetion results in vitro and biodistribution in mice showed that 99m Tc 3H11 and 99m Tc IgG were stable in vitro and in vivo. |
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| Keywords: | m Tc monoclonal antibody direct labelling method biodistribution |
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