| 紫菜叶状体DNA的提取方法 |
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| 引用本文： | 王娟.紫菜叶状体DNA的提取方法[J].菏泽学院学报,2007,29(2):84-87,136. |
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| 作者姓名： | 王娟 |
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| 作者单位： | 菏泽学院生命科学系,菏泽,274015 |
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| 基金项目： | 国家“863”高技术研究发展项目(2002AA6030) |
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| 摘 要： | 为得到适合不同试验的高纯度紫菜DNA,对紫菜叶状体DNA的3种提取方法进行了分析比较.结果显示:酶解法提取的DNA样品质量最高,但要求样品量较多;改良的CTAB法提取的DNA样品多糖含量少,完全可以满足通常的遗传操作的需要,且需要样品量较少;常规的CTAB法提取效果最差.
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| 关 键 词： | 紫菜 叶状体 DNA CTAB 酶解法 |
| 文章编号： | 1673-2103（2007）02-0084-04 |
| 收稿时间： | 2006-09-28 |
| 修稿时间： | 2006-09-28 |
Methods of DNA Extraction from the Leafy Thalli of Porphyra |
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| WANG Juan.Methods of DNA Extraction from the Leafy Thalli of Porphyra[J].Journal of Heze University,2007,29(2):84-87,136. |
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| Authors: | WANG Juan |
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| Institution: | Department of Life Sciences, Heze University, Heze, Shandong 274015, China |
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| Abstract: | In order to obtain Porphyra DNA with high-purity for different experiments,three DNA extraction methods from the leafy thalli of Porphyra are compared.Results showed that DNA extracted with enzymatic isolation is best among the three methods,but more materials are needed.DNA extracted with improved CTAB is good with lesser polysaccharide,which is suited for normal genetic works.Lesser materials are also needed.The routine CTAB gives the worst results among the three methods. |
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| Keywords: | Porphyra thallus DNA CTAB enzymatic isolation |
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