E-cadherin and plakoglobin recruit plakophilin3 to the cell border to initiate desmosome assembly |
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Authors: | Prajakta?Gosavi Samrat?T?Kundu Nileema?Khapare Lalit?Sehgal Mansi?S?Karkhanis Email author" target="_blank">Sorab?N?DalalEmail author |
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Institution: | (1) KS215, Advanced Centre for Treatment Research and Education in Cancer, Tata Memorial Centre, Kharghar Node, Navi Mumbai, 410210, India; |
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Abstract: | A decrease in the levels of the desmosomal plaque protein, plakophilin3 (PKP3), leads to a decrease in desmosome size and
cell-cell adhesion. To test the hypothesis that PKP3 is required for desmosome formation, the recruitment of desmosomal components
to the cell surface was studied in the PKP3 knockdown clones. The PKP3 knockdown clones showed decreased cell border staining
for multiple desmosomal proteins, when compared to vector controls, and did not form desmosomes in a calcium switch assay.
Further analysis demonstrated that PKP3, plakoglobin (PG) and E-cadherin are present at the cell border at low concentrations
of calcium. Loss of either PG or E-cadherin led to a decrease in the levels of PKP3 and other desmosomal proteins at the cell
border. The results reported here are consistent with the model that PG and E-cadherin recruit PKP3 to the cell border to
initiate desmosome formation. |
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