| 基于纳米金探针的光度法测定痕量6-巯基嘌呤 |
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| 引用本文： | 邱小香.基于纳米金探针的光度法测定痕量6-巯基嘌呤[J].河南科学,2014(12):2477-2480. |
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| 作者姓名： | 邱小香 |
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| 作者单位： | 渭南师范学院化学与生命科学学院，陕西渭南 714099; 陕西省多河流湿地生态环境重点实验室，陕西渭南 714099 |
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| 基金项目： | 渭南市科技创新扶持资金项目(2012KYJ-3) |
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| 摘 要： | 在p H 5.5的B-R缓冲溶液中,6-巯基嘌呤与纳米金反应后,纳米金粒子溶液的颜色由酒红色逐渐变为蓝色,其最大吸收波长从525 nm移至670 nm.670 nm和525 nm处吸光度的比值(A670/A525)与6-巯基嘌呤的浓度呈正比.据此,在优化实验条件下,建立纳米金分光光度法测量痕量6-巯基嘌呤的新方法 .该方法准确度高、灵敏度好.
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| 关 键 词： | 6-巯基嘌呤 金纳米粒子 分光光度法 |
Determination of Trace 6-Mercaptopurine Using Nano-gold Spectrophotometric Method |
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| Qiu Xiaoxiang.Determination of Trace 6-Mercaptopurine Using Nano-gold Spectrophotometric Method[J].Henan Science,2014(12):2477-2480. |
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| Authors: | Qiu Xiaoxiang |
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| Institution: | Qiu Xiaoxiang (1. College of Chemistry and Life sciences, Weinan Normal University, Weinan 714099, Shaanxi China 2. Shaanxi River Wetland Ecological Environment Key Laboratory, Weinan 714099, Shaanxi China) |
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| Abstract: | In the B-R pH 5.5 buffer solution,6-mercaptopurine（6-MP）and gold nanoparticles after reaction,goldnanoparticle solution color changed from red to blue,the maximum absorption wavelength redshift from 525 nm to 670 nm. The ratios of 670 nm and 525 nm absorbance（A670/A525）was proportional to the concentration of 6-mercaptopurine.Accordingly,under the optimized experimental conditions,a new method of trace 6-mercaptopurine was establishedwith nano-gold photometric method. The method was simple,accurate and sensitive. |
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| Keywords: | 6-mercaptopurine gold nanoparticles spectrophotometry |
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