葎草花粉主要过敏原的分离鉴定与纯化

采用液氮研磨法提取葎草花粉粗蛋白,凝胶电泳(SDS-PAGE)分离粗提液蛋白组分并测定各组分的相对分子质量,后经过离子交换层析法分离纯化几类主要的蛋白,再用westen blotting分别检测其与花粉过敏患者血清IgE结合情况,鉴定每种主要蛋白质致敏原的致敏性.分析了葎草花粉变应原成分及其变应原性、免疫原性,并鉴定主要过敏原的致敏性.SDS-PAGE凝胶电泳结果表明,广泛的分布于8～170 kDa之间有主带13条,次带10余条;westen blotting检测发现葎草花粉的致敏性较强,与花粉过敏患者血清IgE结合能力较高.离子交换层析出4种主要变应原蛋白,其相对分子质量分别为55,16,15和25 kDa,其中结合能力最强的是15 kDa处蛋白条带.葎草的主要过敏原为55,16,15和25 kDa蛋白质组分.

The Characterization for the Antigenic Properties of Pollen Allergens in Humulus scandens Lour

To characterize and purify the pollen allergens isolated from Humulus scandens Lour,identified the allergenicity of these main allergen.Method:The pollen of Humulus scandens Lour were grinded in liquid nitrogen to extract the total protin,analyzed total protin with SDS-PAGE,detective the proteins' allergenicity with westen-blotting,Ion-exchange chromatography with DE-52 was used for the purification of the antigens.Result:For the pollen extract of Humulus scandens Lour,30 protein bands were detected in SDS - PAGE.Among them,weights bewteen 10-100 kDa has 13 main protiens,more than 10 kinds of secondary protiens.Fore of these protein bands,with a molecular weight of 55 kD,6 kD and 15 kD,25 kD,showed immunoreactivity with IgE in the sera from patients with allergy to pollen of Humulus scandens Lour.Conclusion:Pollen allergens from Humulus scandens Lour were purified and characterized.The result will provide a theoretical foundation for the diagnosis and treatment of allergy to the pollen allergens from Humulus scandens Lour.