| 检测丽蚜小蜂取食寄主银叶粉虱的多重聚合酶链式反应 |
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| 引用本文： | 盖宇 Stout.,R. 检测丽蚜小蜂取食寄主银叶粉虱的多重聚合酶链式反应[J]. 北京师范大学学报(自然科学版), 1999, 35(3): 415-418 |
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| 作者姓名： | 盖宇 Stout. R |
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| 作者单位： | 北京师范大学生命科学学院 |
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| 摘 要： | 结合Ｄ２引物和ＢＥＦ引物，优化了一个可以用来检测丽蚜小峰（Ｅｎｃａｒｓｉａ ｆｏｒｍｏｓａ Ｇａｈａｎ）取食寄主银叶粉虱（Ｂｅｍｉｓｉａ ａｒｇｅｎｔｉｆｏｌｉｉ Ｂｅｌｌｏｗｓ＆Ｐｅｒｒｉｎｇ）的重多聚合酶链式反应。特异于银叶粉虱的ＢＥＦ引物（正向５′－ＡＡＴ ＴＣＡ ＣＡＣ ＡＴＡ ＣＧＴ ＴＡＧ ＣＣＣ ＣＴ－３′和反向５′－ＴＡＡ ＣＣＧ ＡＡＣ ＣＡＴ ＣＡＡ ＣＡＧ ＡＴＴ ＡＴＴ－３
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| 关 键 词： | 丽蚜小蜂 银叶粉虱 寄主取食 粉虱 PCR 生物防治 |
A MULTIPLEX PCR TO DETECT ENCARSIA FORMOSA HOST-FEEDING ON BEMISIA ARGENTIFOLII |
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| Gai Yu, Xu Rumei, Richard Stouthamer, Joop C. van Lenteren. A MULTIPLEX PCR TO DETECT ENCARSIA FORMOSA HOST-FEEDING ON BEMISIA ARGENTIFOLII[J]. Journal of Beijing Normal University(Natural Science), 1999, 35(3): 415-418 |
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| Authors: | Gai Yu Xu Rumei Richard Stouthamer Joop C. van Lenteren |
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| Abstract: | A multiplex PCR, with primers of D2 and BEF, was optimized to detectEncarsia formosa host- feeding on Bemisia argentifolii. The BEF primers (forward: 5' -AAT TCA CAC ATA CGT TAG CCC CT- 3' and reverse: 5'- TAA CCG AAC CTA ACA CAG ATT ATh- 3'), specific to Bemisia argentifolii, was obtained in the amplified fragment length polymorphism analysis of Encarsia formosa which had host- fed on Bemisia argentifolii. |
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| Keywords: | Encarsia formosa Bemisia argentifolii host-feeding multiplex PCR |
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