单纯疱疹病毒抗体的酶联免疫吸附——单扫描示波极谱法测定

报道了用辣根过氧化物酶为标记酶的ELISA—LSP法测定单纯疱疹病毒抗体(HSV—Ab).研究了测定的最适条件;测定了型共同性单克隆抗体,其灵敏度为ELISA的2倍,线性范围比ELISA宽2个滴度;对40个杂交瘤细胞系进行单克隆筛选,测出的阳性克隆率(75%)高于ELISA所测值(72.5%);对阳性克隆的分型结果与ELISA一致,此法灵敏、可靠、易实现.

Determination of Herpes Simplex Virus Antibody (HSV-Ab) by ELISA-LSP and Its Application in the Selection of Anti-HSV Monoclonal Antibodies

: It's reported that hexpes simplex virus-antibody (HSV-Ab) was determined by ELISA-LSP which used HRP as labelling enzyme. The optimum conditions for the determintion were studied. Under optimum conditions,the linear range of HSV-Ab by ELISA-LSP was determined as 1:25 to 1 : 1600 with r=0. 9814, which was higher than 1: 50 to 1 : 800 with r = 0. 9761 by ELISA. The determination limit of ELISA-LSP(1 : 3200) was two times lower than that of ELISA(1 : 1600). The selection of anti-HSV monoclonal antibodies out of 40 systems of hybrid tumor cells was made. The positive clonal ratio was 75% by ELISA-LSP, which was higher than that by ELISA. The results of type identification of positive clonals by the above two methods are in good agreement.