坛紫菜的细胞发育研究

报道了坛紫菜离体单细胞的培养结果．实验表明，单离细胞能在琼脂固体培养基上良好地存活、分裂和再生．正常的体细胞能再生为正常苗、畸形苗、壳孢子以及较多的细胞团等；固体培养下有些细胞团能长成类愈伤组织；一些单离细胞能直接成为壳孢子囊枝；成熟的细胞团和类愈伤组织还能释放出小孢子．

Studies on the solid culture of somatic cells from porphyra haitanensis

This paper reports the result of cultivation of somatic cells from porphyra haitanensis,one of the most important economical seaweeds cultivated in South Coastal of China,selected as the material in research.The result of experiments shows that the isolated nori cells can survive,divide and regenerate well on agar solidified seawater medium.The division and regeneration of nori cells on solid medium is much the same as that in liquid medium in certain extent.The isolated nori cells can form normal thalli,abnormal thalli(with or without holdfast),conchocelis and more cell masses.On solid medium,regenerated thalli can slowly and continuously grow,and conchocelis can normally grow and mature. Cell masses can develop into callus-like clumps.Then the clumps could differentiate into young plantlets in the optimum growth temperature.Few cells can directly sprout into conchosporangial branch and carpogonia on the mature leafy thallus,and could discharge and germinate into carpospores in the same way as monospore did.It had also been demonstrated that the growth and development of isolated nori somatic cells in differential aged individual thalli were quite different in certain respects.