DART-Orbitrap MS快速筛查血液中的130种毒物

血液样品经乙酸乙酯提取、离心、取上清液5μL筛网进样,采用正离子模式扫描进行实时直接分析离子源-静电场轨道阱高分辨质谱(DART-Orbitrap MS)分析,通过比对130种毒物精确分子质量数据库,建立了血液中毒物快速筛查分析方法.结果表明:130种毒物的检出限(以3倍S/N计算)为0.2~50μg/L,质量精确度均在5×10-6μg以内.该方法样品前处理简单、溶剂消耗少,具有分析时间短、灵敏度高的特点,适用于血液中毒物的快速筛查分析.

Rapid Screening of 130 Toxicants in Blood by Direct Analysis in Real Time-orbitrap High-resolution Mass Spectrometry

Take out the blood samples supernatant 5μL mesh screen injection modules,which has been extracted by ethyl acetate and then centrifuged.130 toxicants were analyzed by direct analysis in real time-orbitrap high-resolution mass spectrometry(DART-Orbitrap MS)in positive mode.By comparing the 130 toxicants accurate mass database,a high-throughput method for the screening of toxicants in blood was developed.The results showed the detection limits of these 130 toxicants ranged from 0.2to 50μg/L and Mass error(<5×10-6μg).The method is simple,rapid,sensitive with a little less solvent consumptive.It is suitable for screening and confirmation of toxicant in blood samples.