大鼠促红细胞生成素受体基因克隆及其在颅脑损伤模型中的表达分析

利用巢式PCR(nest PCR)的方法克隆大鼠促红细胞生成素受体基因.该基因ORF区全长1 524bp,编码507个氨基酸,推测其相对分子质量为55.48ku,等电点5.07.实时荧光定量PCR结果显示:大鼠早期颅脑损伤中,EPOR基因表达呈逐步上升趋势,颅脑损伤后24h表达量达到顶峰,为对照组的6倍.

Cloning and Expression Analysis of Rat Erythropoietin Eeceptor in Brain Injury Model

A full length cDNA of rat erythropoietin receptor gene was isolated by nest PCR method.The ORF of EPOR was 1 524 bp,encodes 507 amino acid residues.The deduced molecular weight and PI was 55.48 ku,and the isoelectric point was 5.07.The realtime PCR result indicated that the expression level of EPOR was enhanced during the early time in rat traumatic brain injury(TBI)model.The EPORexpression level of 24 hafter TBI reached to the peak during the TBI period,and the expression amount was 6times of the control.