| 不同烷基酚诱导大鼠支持细胞膜通透性和细胞外基质分泌的对比研究(英文) |
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| 引用本文： | 龚昳,袁春涛,吴江,屠志刚,韩晓冬. 不同烷基酚诱导大鼠支持细胞膜通透性和细胞外基质分泌的对比研究(英文)[J]. 南京大学学报(自然科学版), 2009, 0(4) |
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| 作者姓名： | 龚昳 袁春涛 吴江 屠志刚 韩晓冬 |
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| 作者单位： | 南京大学医学院免疫与生殖生物学实验室;江苏省分子医学重点实验室; |
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| 基金项目： | National Natural Science Foundation of China(20577019) |
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| 摘 要： | 烷基酚作为一类重要的环境污染物已经对男性生殖产生很大的负面影响.本文的主要目的是对比研究不同烷基酚对雄性大鼠睾丸支持细胞的影响.各种烷基酚,包括甲基酚,丁基酚和壬基酚处理支持细胞后,对比研究它们对支持细胞膜通透性和细胞外基质产生的影响.实验结果表明各类烷基酚在处理12 h内均能诱导细胞膜通透性(以lactate dehydrogenase(LDH)泄漏率表示)增加,并具有一定的剂量依赖效应,其中丁基酚和壬基酚的作用强于甲基酚;当处理时间增加至24 h时,各组LDH的泄漏率均在较低浓度即达到最大(丁基酚和壬基酚5×10-8mol/L,甲基酚10-7mol/L)并不再随浓度增加而增大.此外,各种烷基酚处理均能增加支持细胞层粘连蛋白产生量,并具有一定剂量依赖性,其中丁基酚的刺激作用强于甲基酚和壬基酚.
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| 关 键 词： | 烷基酚 对比 lactate dehydrogenase泄漏 层粘连蛋白 支持细胞 |
Comparative study of different alkylphenol induced effect on cell membrane permeability and extracellular matrix production of rat sertoli cells |
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| Gong Yi,Yuan Chun-Tao,Wu Jiang,Tu Zhi-Gang,Han Xiao-Dong. Comparative study of different alkylphenol induced effect on cell membrane permeability and extracellular matrix production of rat sertoli cells[J]. Journal of Nanjing University: Nat Sci Ed, 2009, 0(4) |
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| Authors: | Gong Yi Yuan Chun-Tao Wu Jiang Tu Zhi-Gang Han Xiao-Dong |
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| Affiliation: | Immunology and Reproductive Biology Laboratory;Jiangsu Key Laboratory of Molecular Medicine Medical School;Nanjing University;Nanjing;210093;China |
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| Abstract: | Alkylphenols(APs) as important environment contaminants have caused disruptive effects on male reproduction.The aim of present study was to compare different alkylphenol-induced effect on rat testicular sertoli cells.Alkylphenols,including p-cresol,4-n-butylphenol and 4-n-nonylphenol,were treated to purify rat testicular sertoli cells,and their effects on cell membrane permeability and extracellular matrix production were studied and made comparison.The results revealed that alkylphenols increased membrane ... |
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| Keywords: | alkylphenols comparison lactate dehydrogenase leakage laminin sertoli cells |
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