| 聚丙烯酰胺凝胶电泳快速显色方法的研究 |
| |
| 引用本文： | 刘美艳,营孙阳,张健,楚素芳. 聚丙烯酰胺凝胶电泳快速显色方法的研究[J]. 实验室科学, 2012, 15(6): 71-73 |
| |
| 作者姓名： | 刘美艳 营孙阳 张健 楚素芳 |
| |
| 作者单位： | 江苏师范大学 生命科学学院,江苏 徐州,221116 |
| |
| 基金项目： | 转基因生物新品种重大专项,江苏高校优势学科建设工程资助项目 |
| |
| 摘 要： | 通过对聚丙烯酰胺凝胶电泳的固定方法、时间、染色液种类、染色方法及蛋白灵敏度的研究,确定了以10%三氯乙酸为固定液,室温固定10min的固定方法;染色液为：考马斯亮蓝G-250、95%乙醇和10%磷酸,染色时间为15min;蛋白质灵敏度与考马斯亮蓝R-250相当,达到0.1μg。
|
| 关 键 词： | 蛋白质 电泳 染色 考马斯亮蓝G-250 |
Study on rapid staining protocols of protein in polyacrylamide gel electrophoresis |
| |
| LIU Mei-yan , YING Sun-yang , ZHANG Jian , CHU Su-fang. Study on rapid staining protocols of protein in polyacrylamide gel electrophoresis[J]. Laboratory Science, 2012, 15(6): 71-73 |
| |
| Authors: | LIU Mei-yan YING Sun-yang ZHANG Jian CHU Su-fang |
| |
| Affiliation: | (School of Life Science,Jiangsu Normal University,xuzhou 221116,China) |
| |
| Abstract: | The fixing methods,fixing time,the kind of dyes,staining protocols and the sensitivity of protein in polyacrylamide gel electrophoresis were studied.The fixative solution was 10% trichloroacetic acid fixing for 10min in the normal temperature.The staining solution was CBB G-250,95% ethanol,10% phosphate and the staining time was 15min.The comparable sensitivity of protein after optimization to CBB G-250 staining,which is reaching 0.1μg. |
| |
| Keywords: | protein electrophoresis staining CBB G-250 |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
