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| 兔成骨细胞的体外培养及生物学行为观察 | |
| 引用本文： | 郭勇,张西正,刘欣,赵云山,李瑞欣,曹仪植.兔成骨细胞的体外培养及生物学行为观察[J].兰州大学学报(自然科学版),2002,38(5):71-75. |
| 作者姓名： | 郭勇 张西正 刘欣 赵云山 李瑞欣 曹仪植 |
| 作者单位： | 1. 军事医学科学院,卫生装备研究所,天津,300161 2. 天津市医药科学研究所,天津,300010 3. 兰州大学,生命科学学院,甘肃,兰州,730000 |
| 基金项目： | 国家自然科学基金 (10 172 0 93)，全军医药卫生“十五”科研基金 (0 1MA0 88)资助项目 |
| 摘 要： | 以一月龄幼兔的颅顶骨为材料 ,采用组织块法 ,进行细胞的原代培养并传代之 .结果显示 :(1)原代细胞具有成骨细胞的形态特征 ,经 6次传代后细胞形态变化不明显 ;(2 )细胞具有较强的碱性磷酸酶 (AL P)活性且稳中有升 ;(3)细胞培养液和细胞内均含有骨钙素 ;(4)连续培养 1个月后 ,成骨细胞有矿化结节形成 ;(5 )骨片经 4次贴壁仍有细胞爬出且细胞形态相同 .该结果表明所培养的细胞具有成骨细胞的生物学行为 ,为骨细胞生物学和骨组织工程的研究提供了一种客观而有效的手段 |
| 关 键 词： | 兔成骨细胞 体外培养 生物学行为 |
| 文章编号： | 0455-2059(2002)05-0071-05 |
| 修稿时间： | 2001年10月25 |
Culture in vitro and observation of osteoblasts derived from rabbit calvarial bone |
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| GUO Yong ,ZHANG Xi-zheng ,LIU Xin ,ZHAO Yun-shan ,LI Rui-xin ,CAO Yi-zhi.Culture in vitro and observation of osteoblasts derived from rabbit calvarial bone[J].Journal of Lanzhou University(Natural Science),2002,38(5):71-75. | |
| Authors: | GUO Yong ZHANG Xi-zheng LIU Xin ZHAO Yun-shan LI Rui-xin CAO Yi-zhi |
| Institution: | GUO Yong 1,ZHANG Xi-zheng 1,LIU Xin 2,ZHAO Yun-shan 1,LI Rui-xin 1,CAO Yi-zhi 3 |
| Abstract: | Rabbit osteoblasts were obtained by explanting the calvarial bone pieces of little rabbit cultured in medium,and the biological behaviors of the osteoblast were observed.The result showed the primary cells were homogenous in appearance and had the morphologic characteristics and, following six passages,the morphology of the cells was confirmed. The cells exhibited high alkaline phosphase activity and secreted osteocalcin. A month later,the mineralized nodules of the cells were found. Two months later,cells still creeped out from the calvarial bone pieces continuously. The study revealed that the cells had some biological behaviors of osteoblast,the culture in vitro provides a useful and simple system for the study of bone biology and bone tissue engineering. |
| Keywords: | osteoblast of rabbit culture in vitro biological behavior |
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