出血病及粘孢子虫病鲫鱼蛋白质变化研究

利用双向电泳方法，以正常鲫鱼为对照，对感染出血病和粘孢子虫病后鲫鱼肌肉、肝脏组织内蛋白质变化作了比较分析．结果表明，正常鱼与病鱼肌肉组织显示出的蛋白质斑点约100个，其中含量较丰富的蛋白质斑点有20余种，以酸性蛋白居多．肝脏组织约有200余种蛋白质斑点存在，其中含量比较丰富蛋白质斑点有30余种，有较为广泛的分子量范围；感染出血病鲫鱼的肌肉组织中诱导出6个新的蛋白质斑点，肝脏中诱导出19个蛋白质斑点．在感染粘孢子虫病的鲫鱼肌肉中，有3个蛋白质斑点消失，诱导出3个蛋白质斑点，肝脏中有16个斑点消失，这些变化可以作为辅助疾病诊断的生化指标，为从生化水平揭示该病的致病机理及开展早期预防研究奠定基础．

Studies on the Protein Changes in Hemorrhagic and Myxosporean Diseased Crucian Carp

In contrast with the normal group,the protein changes of muscle and liver tissue for C.auratus var Hemorrhagic-,Myxosprean-infected were analyzed by IEF-PAGE.The results showed that about 100 protein spots were identified in the muscle of normal and Hemorrhagic-,Myxosprean-infected C.auratus var,more than 20 protein spots are abundant in content,most of them are located on the acid region.More than 200 protein spots are identified in the liver tissue of normal and Hemorrhagic-,Myxosprean-infected C.auratus var,about 30 protine spots are abundant in protein content.There is extensive molecular weight scope.6 new protein spots were induced in the muscle and 19 new protein spots were induced in the liver of Hemorrhagic-infected C.auratus var.3 protein spots were disappeared and 3 new protein spots were induced in the muscle,16 protein spots were disappeared in the liver of Myoxsporean-infected C.auratus Var.The protein changes may be considered as a biochemical diagnosis index and the basis for the study of the pathogenic mechanism as well as for the early disease prevention.