固相微萃取-气相色谱/质谱分析生姜的挥发性成分

采用固相微萃取(SPME)技术结合气相色谱—质谱(GC—MS)分析了生姜中的挥发性化合物，用膜厚为100μm的聚二甲基硅氧烷纤维头萃取新鲜生姜中的挥发性化合物，纤维头在温度为250℃气化室进样口脱附3min，通过顶空SPME-GC-MS方法从生姜中共分离和鉴定出36个化合物，其中主要的化合物为α—水芹烯，莰烯，里哪醇，香叶醛，姜烯，倍半水芹烯，橙花醛，α—没药烯，α—姜黄烯，由色谱峰的峰面积比例计算出各个化合物的相对含量，研究和讨论了影响SPME条件的参数，包括吸附时间、温度、脱附时间等。

Gas Chromatography-mass Spectrometry Coupled with Solid-phase Microextraction for Determination of Volatile Constituents of Chinese Ginger

Gas chromatography-mass spectrometry combined with solid-phase microextraction (SPME-GC-MS) was developed for the determination of volatile compounds of ginger. The volatile constituents of fresh ginger were extracted by SPME with a 100 μm polydimethylsiloxane fiber. The fibers were desorbed in a GC injection liner at 250 ℃ for 3 min. With headspace SPME-GC-MS analysis, thirty-six peaks in ginger were separated and identified by mass spectrometry. The volatile compounds included α-phellandrene, camphene, linalool, geranial, zingiberene, sesquiphellandrene, neral, α-bisabolene, α-curcumene etc and their relative contents were calculated on basis of peak area ratio. SPME extraction conditions including time, temperature and desorption time were investigated.