| 介孔SiO2固定化漆酶降解2,4DCP |
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| 引用本文： | 杨宇翔,张亚妮,邵建国,陈娅如.介孔SiO2固定化漆酶降解2,4DCP[J].同济大学学报(自然科学版),2011,39(9):1324-1328. |
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| 作者姓名： | 杨宇翔 张亚妮 邵建国 陈娅如 |
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| 作者单位： | 1. 华东理工大学化学与分子工程学院,上海,200237
2. 扬州大学化学化工学院,江苏扬州,225002 |
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| 基金项目： | 国家自然科学基金(No.20971043和No. 20577010) 资助项目 |
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| 摘 要： | 以M-NH2-SiO2为载体,戊二醛为交联剂制备固定化漆酶,研究2,4-DCP(2,4-二氯苯酚)浓度、溶液酸度、温度对固定化漆酶降解2,4-DCP的影响.结果表明,固定化漆酶降解2,4-DCP最佳条件为:当2,4-DCP质量浓度为5mg.L-1,溶液pH为5.5,温度为30℃时,降解去除率为42.28%.固定化漆酶具有最大活性时的溶液pH值相比于自由酶的最佳pH向碱性偏移;与自由漆酶相比,固定化漆酶有良好的稳定性和重复使用性.
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| 关 键 词： | 介孔SiO2 固定化漆酶 降解率 重复使用性 |
| 收稿时间： | 5/5/2010 6:13:13 PM |
| 修稿时间： | 9/20/2010 6:45:54 PM |
Degradation of 2,4DCP by Immobilized Laccase on Mesoporous Silica |
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| YANG Yuxiang,ZHANG Yani,SHAO Jianguo and CHEN Yaru.Degradation of 2,4DCP by Immobilized Laccase on Mesoporous Silica[J].Journal of Tongji University(Natural Science),2011,39(9):1324-1328. |
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| Authors: | YANG Yuxiang ZHANG Yani SHAO Jianguo and CHEN Yaru |
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| Institution: | YANG Yuxiang1,ZHANG Yani1,SHAO Jianguo2,CHEN Yaru1 (1.College of Chemistry and Molecular Engineering,East China University of Science & Technology,Shanghai 200237,China,2.College of Chemistry & Chemical Engineering,Yangzhou University,Yangzhou 225002,China) |
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| Abstract: | Laccase was immobilized on M-NH2-SiO2 with glutaraldehyde as crosslinking agent.The effects on the activity of immobilized laccase,such as concentration of 2,4-DCP(2,4-dichlorophenols),acidity of solution,temperature were studied.The results show when concentration of 2,4-DCP is 5 mg·L-1,pH of solution is 5.5,and the temperature is at 30℃,the highest removal rate of 2,4-DCP is 42.28%.The results show that the optimal pH for immobilized laccase is higher than the best pH for free enzyme.Compared with the free enzyme,its stability and repetition are considerably improved. |
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| Keywords: | mesoporous SiO2 immobilized laccase degradation rate repetition |
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