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人组氨酰-tRNA合成酶基因的克隆与表达
引用本文:毕智丽,吴亦红,赵晓瑜,倪志华,王三元. 人组氨酰-tRNA合成酶基因的克隆与表达[J]. 河北大学学报(自然科学版), 2005, 25(3): 318-322. DOI: 10.3969/j.issn.1000-1565.2005.03.019
作者姓名:毕智丽  吴亦红  赵晓瑜  倪志华  王三元
作者单位:1.河北大学,生命科学学院,河北,保定,071002; 2.河北省环境科学研究院,河北,石家庄,050051
摘    要:用RT-PCR方法从人胎盘总RNA中获得编码人组氨酰-tRNA合成酶(Jo-1)基因的全序列,选用IMPACT-CN系统中的pTYB11,构建克隆与表达载体,转化大肠杆菌ER2566.经筛选与鉴定,得到3个阳性重组子,对其进行诱导表达获得Jo-1融合蛋白,western-blotting检验结果显示,该融合蛋白具有Jo-1免疫原性和免疫特异性.

关 键 词:人组氨酰-tRNA合成酶  基因克隆  表达  自身抗原  
文章编号:1000-1565(2005)03-0318-05
修稿时间:2004-10-20

Cloning and Expression of Human Histidyl-tRNA Synthetase
BI Zhi-li,WU Yi-hong,ZHAO Xiao-Yu,NI Zhi-hua,WANG San-yuan. Cloning and Expression of Human Histidyl-tRNA Synthetase[J]. Journal of Hebei University (Natural Science Edition), 2005, 25(3): 318-322. DOI: 10.3969/j.issn.1000-1565.2005.03.019
Authors:BI Zhi-li  WU Yi-hong  ZHAO Xiao-Yu  NI Zhi-hua  WANG San-yuan
Affiliation:BI Zhi-li1,WU Yi-hong2,ZHAO Xiao-yu1,NI Zhi-hua1,WANG San-yuan1
Abstract:Using RT-PCR technology the full length DNA sequence coding for human histidyl-tRNA synthetase was obtained. The pTYB11 was used in construction of expressing vector. The recombinant plasmid constructed was transformed into ER2566 and the system was used to express fusion Jo-1 antigen. At last, the author got 3 recombinants that could successfully express the fusion protein. Meanwhile, the expressed fusion protein could be identified by western-blotting using anti-Jo-1 antibody.
Keywords:human histidyl-tRNA synthetase  gene cloning  expression  autoantigen
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