| 两种不同花色矮牵牛快繁体系的建立 |
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| 引用本文： | 李海权,石少华,苏一兰,夏伟婕,李建粤. 两种不同花色矮牵牛快繁体系的建立[J]. 上海师范大学学报(自然科学版), 2008, 37(2): 189-193 |
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| 作者姓名： | 李海权 石少华 苏一兰 夏伟婕 李建粤 |
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| 作者单位： | 上海师范大学,生命与环境科学学院,上海,200234;上海师范大学,生命与环境科学学院,上海,200234;上海师范大学,生命与环境科学学院,上海,200234;上海师范大学,生命与环境科学学院,上海,200234;上海师范大学,生命与环境科学学院,上海,200234 |
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| 摘 要： | 为了建立紫红花和紫花矮牵牛诱导不定芽途径，以这两种矮牵牛为材料，在MS基本培养基基础上，添加不同浓度的6-BA和IBA激素，共设计了9种培养基组合，对两种花色矮牵牛叶片进行快繁研究．试验结果表明：不同品种矮牵牛快繁的激素组合是有差异的，紫红花矮牵牛叶片诱导不定芽的最适培养基为MS4-1．0mg／L6-BA4-0．2mg／LIBA，紫花矮牵牛叶片诱导不定芽最适培养基为MS＋2．0mg／L6-BA＋0．5mg／LIBA．为今后以紫红花和紫花矮牵牛为受体进行遗传转化获得转基因植株的无性快繁建立有效再生体系．
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| 关 键 词： | 紫红花矮牵牛 紫花矮牵牛 叶片 不定芽 快繁体系 |
| 文章编号： | 1000-5137(2008)02-0189-05 |
| 修稿时间： | 2007-12-01 |
Establishment of fast regeneration system for mauve and purple Petunia hybrida |
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| LI Hai-quan,SHI Shao-hua,SU Yi-lan,XIA Wei-jie,LI Jian-yue. Establishment of fast regeneration system for mauve and purple Petunia hybrida[J]. Journal of Shanghai Normal University(Natural Sciences), 2008, 37(2): 189-193 |
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| Authors: | LI Hai-quan SHI Shao-hua SU Yi-lan XIA Wei-jie LI Jian-yue |
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| Affiliation: | (College of Life and Environment Sciences ,Shanghai Normal University, Shanghai 200234, China) |
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| Abstract: | In order to establish a fast regeneration method by buds induction directly for mauve and purple Petunia hybrida, nine kinds of medium combinations on MS basic medium with different concentration of 6 - BA and IBA were designed, and the leaves of two kinds of Petunia hybrida as explants were studied. Result showed that there were obvious differences in the requirement of hormone concentration on fast regeneration between the two Petunias hybrida, the most suitable culture medium was MS ＋ 1.0mg/ L 6 - BA ＋0.2mg/L IBA for mauve Petunia hybrida, and MS ＋ 2.0mg/L 6 - BA ＋ 0.5mg/L IBA for purple Petunia hybrida. This research sets up the available system of fast regeneration for transgenic mauve and purple Petunia hybrida. |
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| Keywords: | mauve Petunia purple Petunia leaf adventitious bud fast regeneration system |
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