| 泸型大曲中根霉固态发酵产糖化酶条件研究 |
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| 引用本文： | 黄丹,尚志超,袁先铃,李玉龙. 泸型大曲中根霉固态发酵产糖化酶条件研究[J]. 四川理工学院学报(自然科学版), 2012, 25(3): 9-13 |
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| 作者姓名： | 黄丹 尚志超 袁先铃 李玉龙 |
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| 作者单位： | 四川理工学院生物工程学院,四川自贡,643000 |
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| 基金项目： | 基金项目:川菜发展研究中心项目，泸州老窖科研基金项目，酿酒生物技术及应用四川省重点实验室项目，四川理工学院研究生创新基金项目 |
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| 摘 要： | 对泸型大曲中根霉菌株固态发酵产糖化酶的条件进行了研究。以糖化酶活力为评价指标,设计单因素实验,研究培养基水分含量、培养时间、培养温度对根霉产糖化酶的影响,在此基础上,利用Box-Benhnken Design的方法,对根霉菌株固态发酵产糖化酶的条件进行优化,结果显示泸型大曲中根霉菌株固态发酵产糖化酶的优化条件为培养温度29℃,培养基水分含量53%,培养时间164h,糖化酶的酶活力为2 194.44 U/g。
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| 关 键 词： | 根霉 糖化酶 固态发酵 产酶条件多相流 |
Study on the Conditions of Glucoamylase Production of the Rhizopus Oryzae in Luzhou-flavor Daqu by Solid State Fermentation |
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| Abstract: | The conditions of glucoamylase production of the Rhizopus oryzae in Luzhou-flavor Daqu by solid state fermentation are studied.By single factor experiment and saccharifying enzymic activityas as an assessing index,the influence on saccharifying enzymic activityas of the water content in culture medium,the culture time,the culture temperature are studied.According to the Box-Benhnken design,the conditions of glucoamylase production of the Rhizopus oryzae are optimized by solid state fermentation.The results show that the optimum conditions of glucoamylase production of the Rhizopus oryzae in Luzhou-flavor Daqu by solid state fermentation are the culture temperature 29℃,the water content in culture medium 53%,culture time 164h,glucoamylase activity was 2 194.44U/g. |
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| Keywords: | Rhizopus oryzae glucoamylase solid state fermentation conditiones of glucoamylase production |
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