| 红霉素产生菌的诱变及培养基优化研究 |
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| 引用本文： | 左勇,李杨,任永利,鞠帅,谢晖,祁峰. 红霉素产生菌的诱变及培养基优化研究[J]. 四川理工学院学报(自然科学版), 2012, 0(1): 14-18 |
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| 作者姓名： | 左勇 李杨 任永利 鞠帅 谢晖 祁峰 |
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| 作者单位： | 四川理工学院生物工程学院 |
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| 摘 要： | 以红色糖多孢菌08L作为出发菌株进行诱变,选育高产菌株。结果表明,诱变得到的UL5菌株发酵水平比出发菌株提高12.6%。再通过培养基优化,其组成为淀粉3%,糊精3%,葡萄糖2%,黄豆饼粉3%,玉米浆1%,硫酸铵0.15%,碳酸钙0.6%,磷酸二氢钾0.02%的条件下,UL5菌株在诱变的基础上,发酵水平再提高15.2%。
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| 关 键 词： | 红色糖多孢菌 诱变 正交实验 发酵 |
Study on the Mutation of Erythromycin Productive Strain and Its Fermentation |
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| ZUO Yong,LI Yang,REN Yong-li,JU Shuai,XIE Hui,QI Feng. Study on the Mutation of Erythromycin Productive Strain and Its Fermentation[J]. Journal of Sichuan University of Science & Engineering(Natural Science Editton), 2012, 0(1): 14-18 |
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| Authors: | ZUO Yong LI Yang REN Yong-li JU Shuai XIE Hui QI Feng |
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| Affiliation: | (School of Biotechnology Engineering,Sichuan University of Science & Engineering,Zigong 643000,China) |
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| Abstract: | The Saccharopolyspora erythraea 08L is used as the starting strain.Processed by ultraviolet mutagenesis,the fermentation product of the mutated strains was increased by 12.6%.In addition,under the optimized culture medium which consisted of starch 3%,dextrin 3%,glucose 2%,soybean meal 3%,corn steep liquor 1%,(NH4)2SO4 0.15%,CaCO30.6%,KH2PO40.02%,the fementation yield of UL5 strains was increased by 15.2%. |
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| Keywords: | Saccharopolyspora erythraea mutagenesis orthogonal test fermentation |
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