| 棉花DNA的提取及其SSR分子标记体系的建立 |
| |
| 引用本文： | 田海燕,田新惠,李艳军,孙杰.棉花DNA的提取及其SSR分子标记体系的建立[J].石河子大学学报,2007,25(2):150-152. |
| |
| 作者姓名： | 田海燕 田新惠 李艳军 孙杰 |
| |
| 作者单位： | 新疆兵团绿洲生态农业重点实验室，新疆兵团绿洲生态农业重点实验室，石河子大学农学院，石河子大学农学院 新疆石河子832003，石河子大学农学院，新疆石河子832003，新疆石河子832003，石河子大学农学院，新疆石河子832003，新疆石河子832003，新疆石河子832003 |
| |
| 基金项目： | 新疆生产建设兵团科技局博士资金资助项目 |
| |
| 摘 要： | 针对棉花富含酚类、多糖等次生物质的特点,建立了一种简便、快速的提取高纯度棉花基因组DNA的方法。同时建立了适合棉花SSR标记的体系和实验方案,并对所构建的QTL定位群体进行了SSR-PCR检测,得到了清晰的多态性SSR标记,为SSR分子标记技术在棉花育种中的应用打下了基础。
|
| 关 键 词： | 棉花 DNA提取 |
| 文章编号： | 1007-7383（2007）02-0150-03 |
| 修稿时间： | 2007-02-10 |
Isolation of Genetic DNA and Establishment of SSR Reaction System in Cotton |
| |
| TIAN Hai-yan,TIAN Xin-hui,LI Yan-jun,SUN Jie.Isolation of Genetic DNA and Establishment of SSR Reaction System in Cotton[J].Journal of Shihezi University(Natural Science),2007,25(2):150-152. |
| |
| Authors: | TIAN Hai-yan TIAN Xin-hui LI Yan-jun SUN Jie |
| |
| Institution: | 1. Key Laboratory of Oasis Ecology Agriculture of Xinjiang BINGTUAN, Shihezi, Xinjiang 832003, China;2. College of Agriculturel, Shihezi University, Shihezi, Xinjiang 832003, China |
| |
| Abstract: | A simple,rapid genetic DNA extraction procedure by improving the CTAB(cetyltrimethyl ammonium bromide) method has been established due to high quantity of phenol and polysaccharide contained in cotton,thus genetic DNA of cotton with high purity can be obtained.The optimized system and experimental program suitable for SSR markers in cotton has also been established,based on which constructed QTL map groups were tested by SSR-PCR.The clear SSR markers with rich polymorphism have been obtained,which will establish the foundation for the application of SSR-based molecular markers on cotton breeding. |
| |
| Keywords: | SSR |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
