Regulations of thyroid hormone on cardiac protein kinase C signal pathway in vitro |
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Authors: | Wang Bao-hua Ouyang Jing-ping Liu Yong-min Zheng Han-qiao and Lin Yin-ping |
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Institution: | (1) School of Medicine, Wuhan University, 430071 Wuhan, Hubei, China |
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Abstract: | The experiments were conducted to assess the influences of thyroid hormone on cardiac protein kinase C(PKC) signal pathway
with cultured cardiac myocytes and fibroblasts as the models. Cells were pretreated with 1% newborn calf serum (NCS) or angiotensin
II (Ang II), and then following by a triiodothyronine (T3) treatment. The PKC activity, PKCα and PKCε expressions were analyzed and compared. In 1% NCS pretreatment, T3 could inhibit PKC activity and PKCε expression in cardiac myocytes. The AngII pretreatment led to an increase of PKC activity and PKCε expression in cardiac myocytes, and an increase of PKC activity in cardiac fibroblasts. Following by T3 treatment, the increased
PKC activity and PKCε expression in cardiac myocytes were markedly decreased. In conclusion, whether in 1% NCS or in Ang II pretreatment, T3 could
inhibit PKC activity and PKCε expression in cardiac myocytes.
Foundation item: Supported by the Natural Science Foundation of Hubei Province (98091)
Biography: WANG Bao-hua (1974-), female, Ph. D, research direction: cardiovascular pathophysiology. |
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Keywords: | thyroid hormone cardiac myocytes cardiac fibroblasts protein kinase C |
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