| 一株极端耐盐曲霉的分离、 鉴定及生物学特性分析 |
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| 引用本文： | 刘晓丹,刘金亮,魏毅,田玉平,范飞飞,潘洪玉,张世宏.一株极端耐盐曲霉的分离、 鉴定及生物学特性分析[J].吉林大学学报(理学版),2011,49(3):548-553. |
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| 作者姓名： | 刘晓丹 刘金亮 魏毅 田玉平 范飞飞 潘洪玉 张世宏 |
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| 作者单位： | 1. 吉林大学 植物科学学院, 长春 130062； 2. 吉林农业科技学院 生物工程学院, 吉林 吉林 132101 |
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| 摘 要： | 从晒盐场盐池周边的风干野生植被表面分离筛选到1株极端耐盐菌株CCHA, 通过形态观察及ITS序列分析对菌株进行鉴定, 并对其生物学特性、 耐盐性及金属耐受性进行分析. 研究结果表明: 菌株CCHA与 21株己报道的曲霉属菌株同源性为55.9%~100%； 其生长温度范围为25~45 ℃, 最适温度为35 ℃； 生长pH值范围为2.5~12.0, 最适pH值为5.0~8.0； 生长盐度范围为0~31%, 最适盐度为5%； 对Cu2+有较强的耐受性. 鉴定该菌株为曲霉菌（Aspergillus sp.）.
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| 关 键 词： | 耐盐曲霉 分离 鉴定 转录间隔区(ITS) 盐度 pH值 金属耐受性 |
| 收稿时间： | 2010-06-01 |
Isolation, Identification and Biologic Characteristics of an Extreme Halotolerant Aspergillus sp. |
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| LIU Xiao-dan,LIU Jin-liang,WEI Yi,TIAN Yu-ping,FAN Fei-fei,PAN Hong-yu,ZHANG Shi-hong.Isolation, Identification and Biologic Characteristics of an Extreme Halotolerant Aspergillus sp.[J].Journal of Jilin University: Sci Ed,2011,49(3):548-553. |
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| Authors: | LIU Xiao-dan LIU Jin-liang WEI Yi TIAN Yu-ping FAN Fei-fei PAN Hong-yu ZHANG Shi-hong |
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| Institution: | 1. College of Plant Sciences, Jilin University, Changchun 130062, China;2. School of Biological Engineering, Jilin Agricultural Science and Technology College, Jilin 132101, Jilin Province, China |
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| Abstract: | A halo tolerant strain CCHA was isolated from the surface of wild vegetation around saltern, identified by the morphological observation and ITS sequence analysis, and their biological characteristics, salt resistance and metal tolerance were analyzed. The homology of ITS sequence between CCHA and each of other 21 Aspergillus strains which have been reported was 55.9%-100%; growth temperature range of it was from 25 ℃ to 45 ℃, the optimum at35 ℃; growth pH range of it was from 2.5 to 12.0, the optimum at 5.0-8.0; salinity range of it was 0-31%, the optimum at 5%; and the strain exhibited higher tolerance to Cu2+. CCHA strain was identified as Aspergillus sp. |
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| Keywords: | halo tolerant Aspergillus sp isolation identification internal transcribed spacer(ITS) salinity pH value metal resistance |
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