Isolation of a growth-stimulating agent from human skin fibroblast cultures |
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Authors: | A A Hakim |
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Institution: | (1) Departments of Surgery, Physiology and Biophysics, Division of Surgical Oncology, College of Medicine University of Illinois at the Medical Center, 180 Longwood Drive, 60901 Kankakee, Illinois, USA) |
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Abstract: | Summary Cell-free supernatants were harvested from cultures of human skin fibroblasts, were applied on to DEAE-cellulose columns, and the first fraction eluted with phosphate-buffered saline contained the growth-stimulating agent. The eluted fraction was then passed through a series of amicon membranes. After passing through PM-10, the filtrate stimulated growth of bovine vascular endothelial, canine myocardial, and human mammary carcinoma cells. |
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