| 固氮酶反应中铁蛋白与MgATP配位的化学模拟 |
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| 引用本文： | 吴也凡,林国栋.固氮酶反应中铁蛋白与MgATP配位的化学模拟[J].厦门大学学报(自然科学版),1992,31(1):78-82. |
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| 作者姓名： | 吴也凡 林国栋 |
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| 作者单位： | 厦门大学生物学系
(吴也凡,曾定),厦门大学化学系
(林国栋),厦门大学化学系(洪亮) |
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| 摘 要： | 以Fe_4S_4(SCH_2Ph)_4]~(-2)作为氧化态铁蛋白4Fe-4S中心的模型化合物,化学模拟研究表明,ATP能与Fe_4S_4(SCH_2Ph)_4]~(-2)原子簇结合,促进原子簇与亚甲蓝之间的氧化还原反应速率,并敏化原子簇中的Fe(Ⅱ),使其易与亚铁螯合剂反应,用正庚烧作萃取刑,在原子簇-ATP体系没有检测到游离的HSCH_2Ph,ATP没有置换原子簇中的—SCH_2Ph,这表明作为电子活化剂的ATP可能通过其磷酸根与Fe_4S_4原子簇结合。
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| 关 键 词： | MgATP 固氮酶反应 铁蛋白 |
Chemical Modeling of Complexation of Fe-Protein with MgATP in Nitrogenase Reactions |
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| Wu Yefan Zeng Ding Lin Guodong Hong Liang.Chemical Modeling of Complexation of Fe-Protein with MgATP in Nitrogenase Reactions[J].Journal of Xiamen University(Natural Science),1992,31(1):78-82. |
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| Authors: | Wu Yefan Zeng Ding Lin Guodong Hong Liang |
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| Abstract: | Chemical-modeling studies show that ATP can complex with Fe4S4 (SCH2Ph)4]-2 cluster which as a model compound of the 4Fe-4S center of the oxidized Fe-protein,resulting in significant enhancement of redox reaction rates between Fe4S4(SCHzPh)4]-2(Z=2,1) and methylene blue,in dramatic sensitization of Fe4S4(SCHzPh)4]-2 to disruption by iron chelaters,as well as in no free HSCH2Ph could be detected with AgDz-CCl4 (dithiazon reagent) in the n-heptane extracts from Fe4S4(SCH2Ph)4]-2 - ATP systems as addition ligand without displacing any of the thio-ligand. It indicates that ATP which serve as "electron activator" were probably coordinated through the binding of their PO4 group to the cubane cluster. |
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| Keywords: | Fe4S4 cluster ATP Complex |
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