HSVTK gene therapy for carcinoembryonic antigen-producing human lung cancer cells |
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Authors: | Xiao Geng-fu Qi Yi-peng Cheng Xuan-hong Li Ling-yun |
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Institution: | (1) College of Life Sciences, Wuhan University, 430072 Wuhan, China |
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Abstract: | The long-term success of gene therapy for cancer relies heavily on the development of effective targeting systems. We investigate
the possibility of targeted gene therapy using promoter of carcinoembryonic antigen (CEA) gene. By using luciferase reporter
gene, we found that CEA promoter exhibit 16 times high activity in CEA-producing lung cancer cells, A549 than in nonproducing
cells, Hela. We also constructed a recombinant expression plasmid pCEATK, in which CEA promoter drives the effector gene,
thymidine kinase gene of Herpes Simplex Virus (HSVTK). A549 cells transfected with pCEATK became 865 times more sensitive
to ganciclovir (GCV) than the control cells. However, Hela cells transfected with this plasmid remained resistant to GCV.
These data indicate the potential for targeted gene therapy using the CEA promoter against CEA-producing tumor cells, such
as lung cancer cells.
Foundation item: Supported by the National Natural Science Foundation of China Natural Science Foundation of Hubei Province
Biography: XIAO Geng-fu(1966-), male, phD graduate candidate, Lecturer. |
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Keywords: | carcinoembryonic antigen Herpes Simplex Virus thymidine kinase gene gene therapy |
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