| 甘薯类胡萝卜素合成酶基因pds全长cDNA的克隆 |
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| 引用本文： | 王飞. 甘薯类胡萝卜素合成酶基因pds全长cDNA的克隆[J]. 安徽理工大学学报(自然科学版), 2007, 27(1): 55-58 |
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| 作者姓名： | 王飞 |
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| 作者单位： | 安徽理工大学医学院,安徽,淮南,232001 |
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| 摘 要： | 根据双子叶植物类胡萝卜素合成结构基因氨基酸保守区域设计简并引物,扩增出甘薯编码类胡萝卜素合成关键酶PDS的基因(pds)cDNA中间片断。通过5'-RACE和3'-RACE技术进行cDNA末端的快速扩增,成功地克隆了pds的全长cDNA。序列分析表明,pds基因的全长cDNA序列长度为2128 bp,编码572个氨基酸,与其他植物的序列高度同源。
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| 关 键 词： | 甘薯 八氢番茄红素去饱和酶(PDS) cDNA 克隆 |
| 文章编号： | 1672-1098(2007)01-0055-04 |
| 修稿时间： | 2006-03-20 |
Clone of Full-length cDNA of Phytoene Desaturase(Gene pds) Gene in Sweet Potato(Ipomoea Batatas L.) |
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| WANG Fei. Clone of Full-length cDNA of Phytoene Desaturase(Gene pds) Gene in Sweet Potato(Ipomoea Batatas L.)[J]. Journal of Anhui University of Science and Technology:Natural Science, 2007, 27(1): 55-58 |
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| Authors: | WANG Fei |
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| Affiliation: | Medical School, Anhui University of Science and Technology, Huainan Anhui 232001,China |
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| Abstract: | Phytoene desaturase gene(pds) is a major carotene gene.In order to learn its role in sweet potato carotenoid biosynthesis,a full-length cDNA was isolated.Firstly,degenerate primers were designed from the conserved motifs of carotene genes isolated from other dicotyls,and the cDNA fragment of pds was cloned and sequenced.Secondly,5'-and 3'-end of the cDNA was cloned through RACE methods.Finally,fragments were assembled and analyzed.The cDNA sequence,which length was 2 128 bp,had an open reading frame.Based on the deduced amino acid sequence,the sweet potato pds gene was found to have 572 amino acid residues and was significantly conserved with other plants. |
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| Keywords: | sweet potato phytoene desaturase cDNA clone |
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