Alternative split sites for fragment complementation,and glyphosate function as extra ligand and stabilizer for the AroA enzyme complexes |
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| Authors: | Yan Li YiCheng Sun HaiQin Yan YiPing Wang |
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| Affiliation: | LI Yan1,SUN YiCheng1,2,YAN HaiQin1 & WANG YiPing1* 1 National Key Laboratory of Protein Engineering and Plant Genetic Engineering,College of Life Sciences,Peking University,Beijing 100871,China,2 Institute of Pathogen Biology,Chinese Academy of Medical Sciences and Peking Union Medical College,Beijing 100730 |
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| Abstract: | Protein reconstitution analysis can be useful for studies on protein evolution, protein folding and macromolecular assembly. AroA is a key enzyme in the pathway toward the synthesis of aromatic amino acids in microorganisms and plants, and is the target of the herbicide glyphosate. Our previous study showed that functional AroA enzyme from Escherichia coli could be reconstituted from two ~220-amino acid fragments of the protein. In this study, we explored this fragment complementation of AroA. Through a sys... |
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| Keywords: | AroA fragment complementation glyphosate extra ligand complex stability |
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