| 一种新的cDNA克隆的分析及可能功能推测 |
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| 引用本文： | 赵贵军,黄玉珊,向孟清,钟肖芬,徐安龙.一种新的cDNA克隆的分析及可能功能推测[J].中山大学学报(自然科学版),2001,40(2):98-100. |
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| 作者姓名： | 赵贵军 黄玉珊 向孟清 钟肖芬 徐安龙 |
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| 作者单位： | 1. 中山大学生命科学学院,
2. 美国新泽西州州立大学, |
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| 基金项目： | 国家自然科学基金资助项目（39800073） |
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| 摘 要： | 筛选胎儿心脏文库时发现一个新cDNA,Genbank搜索和BLAST表明该断cDNA和蛋白激酶A锚定蛋白AKAP95有71％的蛋白质相似性，且在3′端存在一个锌指结构和AKAP基因特有的RⅡ结合区域，因此初步判定该新cDNA为一种AKAP基因；该cDNA和人类19号染色体的一段区域相同，HGP计划标明在cosmid R33907和cosmid F20900上，而该个粒定位在19号染色体的q13．1处，因此将该新基因定位于人第19号染色体的q13．1处，并且同AKAP95相邻。
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| 关 键 词： | cDNA PKA AKAP BLAST 胎儿心脏文库 蛋白激酶A锚定蛋白 基因克隆 |
| 文章编号： | 0529-6579(2001)02-0098-03 |
Sequence Analysis and Characterization of a Novel cDNA Clone |
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| ZHAO Gui-jun,Huang Yu-shan,XIANG Meng-qing,ZHONG Xiao-fen,XU An-long.Sequence Analysis and Characterization of a Novel cDNA Clone[J].Acta Scientiarum Naturalium Universitatis Sunyatseni,2001,40(2):98-100. |
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| Authors: | ZHAO Gui-jun Huang Yu-shan XIANG Meng-qing ZHONG Xiao-fen XU An-long |
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| Institution: | ZHAO Gui-jun 1,HUANG Yu-shan 1,XIANG Meng-qing 2,Zhong Xiao-fen 1,XU An-long 1 |
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| Abstract: | A novel cDNA was discovered through the screening of a human fetal heart library Genbank searching and blasting suggested that the cDNA sequence probably encodes a novel protein belonging to A-Kinase Anchoring Protein (AKAP) family and its predicted protein sequence shows 71% similarity with AKAP95 RII-binding site in its 3' terminal was also found by Blasting with Genbank database The sequence was found to be included in Cosmid R33907 and F20900 originated from Q13 1 locus in Chromosome 19, suggesting that this gene locate in Q13 1 on Chromosome 19 and interestingly neighbore with AKAP95 |
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| Keywords: | novel cDNA PKA AKAP BLAST |
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