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EST analysis of Prorocentrum donghaiense with emphasis on genes involved in PCD |
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| Authors: | Zhang Xiufang Liu Yongjian Yang Guanpin Zhu Mingyuan Li Ruixiang |
| Affiliation: | 1. College of Marine Life Sciences,Ocean University of China,Qingdao 266003,P.R.China;School of Life Sciences,Shandong University of Technology,Zibo 255049,P.R.China 2. College of Marine Life Sciences,Ocean University of China,Qingdao 266003,P.R.China 3. The First Institute of Oceanography,National Oceanic Administration of China,Qingdao 266061,P.R.China |
| Abstract: | Prorocentrum donghaiense has caused large-scale red tides off the Chinese coast in recent years.Ex- pressed sequence tag(EST)analysis was carried out for this dinoflageilate in order to identify the func- tional genes involved in its biological processes.A cDNA library was constructed for P.donghaiense at exponential growth phase,and 565 usable sequencing reads were obtained from 700 clones selected ran- domly.Messenger RNA corresponding reads were clustered into 36 contigs and 272 singletons(EST groups).Tw... |
| Keywords: | Prorocentrum donghaiense functional gene expressed sequence tag programmed cell death |
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