Production of butyrylcholinesterase by Caco-2 cells: lack of relationship with triglyceride production |
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Authors: | Randell E W Rogers B Smith E |
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Institution: | (1) Department of Laboratory Medicine, Faculty of Medicine, Health Sciences Centre, 300 Prince Philip Dr., St. John's, Newfoundland AIB 3V6 (Canada), Fax +1 709 777 6395, e-mail: erandell@morgan.usc.mun.ca, CA;(2) Department of Biochemistry, Faculty of Science, Memorial University of Newfoundland, Science Building, Elizabeth Ave., St. John's, Newfoundland A1C 5S7 (Canada), CA |
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Abstract: | Elevated levels of butyrylcholinesterase activity occur under a number of hypertriglyceridemic conditions, including diabetes
and obesity. This study examines whether butyrylcholinesterase activity has a direct effect on triglyceride production, using
Caco-2 cells, a human intestinal adenocarcinoma cell line. Caco-2 cells were incubated with 500 μM oleate to stimulate triglyceride
production, and butyrylcholinesterase activity was measured in the cellular homogenate. Butyrylcholinesterase activity was
approximately 3 × 10-3 mmol/min per milligram protein. Although triglyceride production increased by almost five-fold after 18 h of stimulation
with oleate, butyrylcholinesterase activity was not increased. Furthermore, inhibition of butyrylcholinesterase activity using
1 mM tetraisopropylpyrophosphoramide did not significantly affect triglyceride production or secretion. Human insulin (100
μU/ml) increased the production of butyrylcholinesterase without increasing triglyceride production. This demonstrates that
stimulation of fatty acid production and butyrylcholinesterase activity occur by independent mechanisms and suggests that
their correlation in hyperlipidemic conditions is not due to a direct relationship in production in situ.
Received 23 April 2001; received after revision 25 May 2001; accepted 20 June 2001 |
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Keywords: | , Butyrylcholinesterase, cholinesterase, obesity, lipid, triglyceride, insulin, Caco-2, diabetes, |
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