Expression of vascular endothelial growth factor in rat uterus during peri-implantation |
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Authors: | Longjiang Shao Hongmei Wang Enkui Duan Cheng Zhu |
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Institution: | (1) State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, 100080 Beijing, China |
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Abstract: | The first distinct mark of rodent implantation is the increased vascular permeability and significant angiogenesis at the
sites of blastocyst implantation, but its mechanism is not clearly defined. Vascular endothelial growth factor (VEGF) is the
key mediator for angiogenesis during embryogenesis and adult span and also serves as a vascular permeability factor. The aim
of this study is to explore VEGF regulation mechanism and the possible role that VEGF plays in implantation by studying the
VEGF expression and angiogenesis in the rat uterus during estrous cycle, ovarioectomized and peri-implantation stages usingin situ message RNA hybridization and confocal laser scanning techniques. The results indicated that VEGF was regulated by ovarian
steroid hormones. VEGF expression before implantation was localized at luminal epithelium, shifted to stroma as implantation
initiated and extensively located at the decidualizing stroma region after implantation. Bandeiraea simplicifolia-1 (BS-1)
agglutinin and antibody against von Willebrand factor (vWF) were used to mark the endothelial cells and blood vessels. The
results showed that the active angiogenesis occurred during the implantation process and this effect was probably mediated
by VEGF. The results suggest that under the regulation of ovarian steroid hormones, VEGF plays an essential role in angiogenesis
and increasing vascular permeability in endometrium, which are necessary for successful implantation. |
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Keywords: | rat vascular endothelial growth factor embryo implantation angiogenesis |
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