| 抗α毒素单链抗体基因表达载体的构建 |
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| 引用本文： | 赵宝华. 抗α毒素单链抗体基因表达载体的构建[J]. 湖南文理学院学报(自然科学版), 2001, 13(2): 67-69 |
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| 作者姓名： | 赵宝华 |
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| 作者单位： | 河北师范大学生命科学学院!河北石家庄050016 |
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| 摘 要： | 将 2种抗A型产气荚膜梭菌α毒素单链抗体 (ScFv)基因ScFv - 2E3和ScFV - 1A8分别克隆至表达质粒pUC119,pET - 2 0b ,pET - 2 8a和pHOG2 1中 ,构建了重组质粒PUC -2E3和pUC - 1A8,pET2 0b - 2E3和pET2 0b - 1A8,pET2 8a - 2E3和pET2 8a - 1A8以及pHOG - 2E3和pHOG - 1A8,然后分别转化至大肠杆菌中 ,提取质粒 ,并进行酶切鉴定和核苷酸序列分析 ,结果表明构建的重组质粒中均含目的ScFv基因片段 ,说明已成功构建了 8个含ScFv基因的表达质粒
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| 关 键 词： | α毒素 ScFv基因 表达质粒 |
| 文章编号： | 1009-3818(2001)02-0067-03 |
| 修稿时间： | 2001-04-09 |
CONSTRUCTION OF EXPRESSION PLASMID WITH ScFv GENE FOR ANTI-ALPHA-TOXIN |
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| ZHAO Bao-hua. CONSTRUCTION OF EXPRESSION PLASMID WITH ScFv GENE FOR ANTI-ALPHA-TOXIN[J]. Journal of Hunan University of Arts and Science:Natural Science Edition, 2001, 13(2): 67-69 |
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| Authors: | ZHAO Bao-hua |
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| Abstract: | First,the ScFv genes were cleaved with Not I and Sfi I from plasmid pXScFv-2E3 and pXScFv-1A8 containing ScFv gene for anti-apha-toxin of Clostridium perfringens type A,isolated by 1.5% agarose gel electrophoresis,recovered the ScFv gene fragment.Second,the ScFv gene with Nco I and Bam HI was obtained by amplifing from the above ScFv segment.Third,these cleaved ScFv gene fragments were inserted into plsamid pHOG21,pET-20b,pET-28a,pUC119 which cleaved with Not I and Sfi I,Bam HI and Nco I respectively by cohesive-end ligation.The results showed that 8 expression plasmid with ScFv gene were constructed. |
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| Keywords: | anti-alpha-toxin ScFv gene expression piasmid |
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