| 紫花苜蓿转录因子MsDREB1基因表达产物的亚细胞定位 |
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| 引用本文： | 李嘉玮,刘晓颖,王振英. 紫花苜蓿转录因子MsDREB1基因表达产物的亚细胞定位[J]. 天津师范大学学报(自然科学版), 2012, 32(1): 85-87,92 |
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| 作者姓名： | 李嘉玮 刘晓颖 王振英 |
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| 作者单位： | 天津师范大学生命科学学院,天津300387;天津市细胞遗传与分子调控重点实验室,天津300387 |
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| 基金项目： | 天津市科委重点资助项目,天津师范大学市级重点实验室开放研究基金资助项目,天津师范大学实验室改革研究基金资助项目 |
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| 摘 要： | 利用生物信息学方法对紫花苜蓿MsDREBl进行了生物信息学分析．结果表明,该序列舍有AP2典型结构域,在N端存在核定位信号．为进一步验证该基因功能,构建MsDREBl与绿色荧光蛋白（GreenFluorescentProtein,GFP）基因融合的植物表达载体pCAMBIAl302-MsDREBl,再利用基因枪将其转入洋葱表皮细胞,在共聚焦扫描显微镜下观察MsDREBl基因表达产物在洋葱表皮细胞中的亚细胞定位．结果表明,MsDREBl基因表达产物定位于细胞核中,符合DREB家族转录因子特性．
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| 关 键 词： | 紫花苜蓿 MsDREBl基因 GFP表达载体 亚细胞定位 |
Subcellular localization of MsDREB1 gene in alfalfa |
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| LI Jia-wei , LIU Xiao-ying , WANG Zhen-ying. Subcellular localization of MsDREB1 gene in alfalfa[J]. Journal of Tianjin Normal University(Natural Science Edition), 2012, 32(1): 85-87,92 |
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| Authors: | LI Jia-wei LIU Xiao-ying WANG Zhen-ying |
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| Affiliation: | a.College of Life Science,Tianjin Normal University,Tianjin 300387,China; b.Tianjin Key Laboratory of Cyto-Genetical and Molecular Regulation,Tianjin 300387,China |
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| Abstract: | Bioinformatics analysis shows that MsDREB1 gene in alfalfa contains AP2/EREBP DNA domain,and has nuclear localization signal in the N-terminal.In order to further verify the function of this gene,a plant expression vector pCAMBIA1302——MsDREB1 with a green fluorescent protein(GFP) gene has been constructed and then transformed into onion skin cells by gene gun method.The result of transient expression which was showed in the confocal scanning microscope indicated that the MsDREB1 gene products was located in the nucleus which is in line with the characteristics of DREB transcription factor family. |
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| Keywords: | alfalfa(Medicago sativa L.) MsDREB1 gene GFP plant expression vector subcellular localization |
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