一个Ds插入标签基因OsPI—PLC2的分子鉴定及表达分析

在前期构建的水稻转座子标签插入突变体库中，鉴定了一个没有明显表型变化的Ds转座子标签系，发现Ds标签插入到一个编码磷脂酰肌醇磷脂酶C（PI-PLC）基因的第6个内含子中，分析该基因编码的氨基酸序列发现其含有已知的PI—PLC所具有的典型的X、Y和C2-like保守结构域，与已知的其它植物的PI-PLC蛋白有较高的同源性．另外，利用RT—PCR对该基因进行了表达分析，结果表明该基因在正常条件下表达量很低，但在胁迫条件下（机械损伤、盐、干旱、低温）或一些信号分子（SA、ABA）的诱导下大量表达，表明该基因参与植物对不良环境条件的信号转导途径，对于植物抵御不良环境条件具有重要作用．

Characterization and expression analysis of a Ds-tagging line OsPI-PLC2 in rice

A Ds-tagging line OsPI-PLC2 without phenotype change was characterized by rice insertional mutant library.Ds-tag inserted into the sixth intron of OsPI-PLC2 gene which encoded a phosphoinositide-specific phospholipase C protein.The sequence analysis showed that OsPI-PLC2 protein contained typical X,Y and C2-like domains which were very conservative in known PI-PLC protein and had high homology with known PI-PLC proteins of other plants.Moreover,the expression pattern of OsPI-PLC2 gene was further investigated by using RT-PCR,and it was demonstrated that OsPI-PLC2 gene exhibited weak expression in normal condition but exhibited strong expression in wound,salt,dry,cold,salicylic acid(SA) and abscisic acid(ABA) induction conditions,suggesting that OsPI-PLC2 gene played an important role in stress signal transduction pathway and resisting harmful environment.