A novel nonviral nanoparticle gene vector: Poly-L-lysine-silica nanoparticles |
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Authors: | Shiguo Zhu Hongbin Lu Juanjuan Xiang Ke Tang Bicheng Zhang Ming Zhou Chen Tan Guiyuan Li |
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Institution: | (1) Cancer Research Institute, Xiangya School of Medicine, Central South University, 410078 Changsha, China |
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Abstract: | DNA delivery is a core technology for gene structure and function research as well as clinical settings. The ability to safely
and efficiently targeted transfer foreign DNA into cells is a fundamental goal in biotechnology. With the development of nanobiotechnology,
nanoparticle gene vectors brought about new hope to reach the goal. In our research, silica nanoparticles (SiNP) were synthesized
first in a microemulsion system polyoxyethylene nonylphenyl ether (OP-10)/cyclohexane/ammonium hydroxide, at the same time
the effects of SiNP size and its distribution were elucidated by orthogonal analysis; then poly-L-lysine (PLL) was linked
on the surface of SiNP by nanoparticle surface energy and electrostatically binding; lastly a novel complex nanomateial—poly-L-lysine-silica
nanoparticles (PLL-SiNP) was prepared. The analysis of plasmid DNA binding and DNase I enzymatic degradation discovered that
PLL-SiNP could bind DNA, and protect it against enzymatic degradation. Cell transfection showed that PLL-SiNP could efficiently
transfer PEGFPC-2 plasmid DNA into HNE1 cell line. These results indicated that PLL-SiNP was a novel nonviral nanoparticle
gene vector, and would probably play an important role in gene structure and function research as well as gene therapy. |
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Keywords: | nanoparticle gene vector silica nanoparticles poly-L-lysine-silica nanoparticles synthesis DNA delivery cell transfection |
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