Affinity chromatography of glucose dehydrogenase |
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| Authors: | W R Carper W C Groutas D B Coffin |
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| Affiliation: | Department of Chemistry, Wichita State University, Kansas 67208. |
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| Abstract: | Porcine liver beta-D-glucose dehydrogenase, a multi-functional protein, has been purified to apparent homogeneity. The enzyme has been separated from the endoplasmic reticulum using Triton X-114 and further purified using NAD to release glucose dehydrogenase from a NADP-linked sepharose column. The purified enzyme is capable of producing both NADH and NADPH in vivo as indicated by kinetic studies. |
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