Affinity chromatography of glucose dehydrogenase |
| |
| Authors: | W R Carper W C Groutas D B Coffin |
| |
| Institution: | Department of Chemistry, Wichita State University, Kansas 67208. |
| |
| Abstract: | Porcine liver beta-D-glucose dehydrogenase, a multi-functional protein, has been purified to apparent homogeneity. The enzyme has been separated from the endoplasmic reticulum using Triton X-114 and further purified using NAD to release glucose dehydrogenase from a NADP-linked sepharose column. The purified enzyme is capable of producing both NADH and NADPH in vivo as indicated by kinetic studies. |
| |
| Keywords: | |
|
|
