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Identification of in vivo interaction between rabbit hemorrhagic disease virus capsid protein and minor structural protein |
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| Authors: | ZongWei Yang Zheng Ni Tao Yun ZongYan Chen ChuanFeng Li GuangQing Liu |
| Affiliation: | 1. Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai, 200241, China 3. Faculty of Veterinary Medicine, Gansu Agricultural University, Lanzhou, 730070, China 2. Zhejiang Academy of Agricultural Sciences, Hangzhou, 300021, China |
| Abstract: | We investigated the ability of the rabbit hemorrhagic disease virus(RHDV) capsid protein(VP60) to interact specifically with the minor structural protein VP10,using an in vivo cell-based CheckMate Mammalian Two-Hybrid System.RHDV VP60 protein interacted specifically with VP10.Immunofluorescence analysis and co-immunoprecipitation with specific antibodies revealed the existence of biologically important VP60/VP10 complexes.However,when VP60 was divided into two fragments,the interaction between VP60 and VP10 was impaired dramatically.These results will be helpful for further investigating the mechanism of RHDV particle assembly. |
| Keywords: | rabbit hemorrhagic disease virus VP60 VP10 protein-protein interaction |
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