Active transport of [32P]thiamine diphosphate inEscherichia coli |
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| Authors: | T Nishimune R Hayashi |
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| Institution: | (1) Department of Microbiology, Yamaguchi University School of Medicine, 755 Ube, Yamaguchi-ken, Japan;(2) Present address: Ohita Medical College, 879-56 Ohita, Japan;(3) Present address: Yamaguchi University School of Medicine, Yamaguchi, Japan |
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| Abstract: | Summary Active uptake of 32P]thiamine diphosphate byE. coli was analyzed using an improved method of gel filtration chromatography. The radioactive coenzyme was accumulated without dephosphorylation. From this result it was concluded that thiamine kinase is not involved in the membrane transport of thiamine inE. coli.We are indebted to Miss M. Abe for her technical assistance. |
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