| 痢疾杆菌膜表面抗原分析 |
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| 引用本文： | 张兴群,陆学东.痢疾杆菌膜表面抗原分析[J].西北大学学报,1996,26(2):167-169. |
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| 作者姓名： | 张兴群 陆学东 |
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| 作者单位： | 西北大学生物学系，陕西省人民医院老年病研究中心 |
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| 摘 要： | 采用ＳＤＳ－ＰＡＧＥ＃胶电泳和免疫印迹技术（Ｗｅｓｔｅｒｎｂｌｏｔｔｉｎｇｔｅｃｈｎｉｑｕｅ）对痢疾杆菌膜表面抗原进行了分析，测定了主要蛋白的分子量。依电泳凝胶染色和免疫印迹图谱，分析了痢疾菌４个群的主要抗原及福氏菌的群特异性抗原和两个型的型特异性抗原，并比较了志贺氏菌属（Ｓｈｉｇｅｌｌａ）实验选用菌株的抗原蛋白。同时，对几种菌的生长曲线进行了测定。
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| 关 键 词： | SDS—PAGE 免疫印迹技术 群特异性抗原 型特异性抗原 |
The Analysis of the Outer Membrane Antigen of shiglla |
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| Zhang Xingqun, Lu Xuedong, Liu Zeya.The Analysis of the Outer Membrane Antigen of shiglla[J].Journal of Northwest University(Natural Science Edition),1996,26(2):167-169. |
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| Authors: | Zhang Xingqun Lu Xuedong Liu Zeya |
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| Abstract: | The SDS-PAGE electrophoresis and western blotting technique are used to research the outer membrane antigens of some species in Shigella. From the results of the methods. the main membrane antigens of four species in Shigella and group and type specific antigens of Shiglla mxneri are analysed and compared. The molecular weight of membrane proteins and growth curve of the bacteria are also measured. |
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| Keywords: | SDS-PAGE western blotting technique group specific antigen type specific antigen |
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