| 重组人骨形态发生蛋白-2和-6对人骨肉瘤细胞株MG63和U2OS的作用 |
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| 引用本文： | 何焕玲,李星星,吴丽美,陈英华,卫佳,苗静琨,周兰.重组人骨形态发生蛋白-2和-6对人骨肉瘤细胞株MG63和U2OS的作用[J].科技导报(北京),2009,27(17). |
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| 作者姓名： | 何焕玲 李星星 吴丽美 陈英华 卫佳 苗静琨 周兰 |
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| 作者单位： | 重庆医科大学生物医学检验系,临床检验诊断学教育部重点实验室,重庆,400016 |
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| 基金项目： | 重庆市自然科学基金项目 |
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| 摘 要： | 为研究重组人骨形态发生蛋白rhBMP-2和rhBMP-6对人骨肉瘤细胞株MG63和U20S的作用,分别用含rhBMP-2和rhBMP-6及重组绿色荧光蛋白的条件培养液干预人骨肉瘤细胞MG63和U2OS,利用台盼蓝拒染法、TUNEL法、吖啶橙/溴乙啶(AO/EB)荧光双染法、Transwell小窒和碱性磷酸酶活性测定法分别检测细胞增殖、凋亡、迁移以及成骨分化能力的变化.结果显示与未实施任何千预的空白对照组和用rGFP干预实验对照组相比.rhBMP-2和rhBMP-6的干预致两种骨肉瘤细胞株:细胞存活率均随时间逐渐降低;凋亡率呈时间依赖性增加;穿膜数明显减低;碱性磷酸酶(ALP)活性逐渐增加.以上差异均有统计学意义(P＜0.01).表明rhBMP-2和rhBMP-6可抑制人骨肉瘤细胞株MG63和U2OS的增殖和迁移、诱导其凋亡和向成骨细胞分化.
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| 关 键 词： | 骨形态发生蛋白 细胞增殖 细胞凋亡 细胞迁移 碱性磷酸酶活性 |
Effects of Recombinant Human Bone Morphogenetic Protein-2 and -6 on Human Osteosarcoma Cell Lines MG63 and U2OS |
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| HE Huanling,LI Xingxing,WU Limei,CHEN Yinghua,WEI Jia,MIAO Jingkun,ZHOU Lan.Effects of Recombinant Human Bone Morphogenetic Protein-2 and -6 on Human Osteosarcoma Cell Lines MG63 and U2OS[J].Science & Technology Review,2009,27(17). |
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| Authors: | HE Huanling LI Xingxing WU Limei CHEN Yinghua WEI Jia MIAO Jingkun ZHOU Lan |
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| Institution: | HE Huanling,LI Xingxing,WU Limei,CHEN Yinghua,WEI Jia,MIAO Jingkun,ZHOU Lan Department of Medical Laboratry,Key Laboratory of Clinical Laboratory Diagnostics Founded by Ministry of Education,Chongqing Medical University,Chongqing 400016,China |
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| Abstract: | To investigate the effects of recombinant human BMP(rhBMP)-2 and -6 on osteosarcoma in treating human osteosarcoma cell lines MG63 and U2OS with rhBMP-2 and rhBMP-6,the osteosarcoma cells in experimental groups were treated with rhBMPs-CMs,those in control groups with rGFP-CM,and those in blank groups with neither.Cell proliferation,apoptosis transmigration and differentiation were detected at different times by Trypan blue exclusion test,transwell-room,terminal deoynucleotidyl transferase mediated dUTP nic... |
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| Keywords: | bone morphogenetic protein cell proliferation cell apoptosis cell transmigration alkaline phosphatase activity |
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