| 体外酶法水解制备小分子透明质酸 |
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| 引用本文： | 原攀红,康振,金鹏,刘龙,堵国成,陈坚.体外酶法水解制备小分子透明质酸[J].北京工商大学学报(自然科学版),2016,34(2):51-55, 82. |
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| 作者姓名： | 原攀红 康振 金鹏 刘龙 堵国成 陈坚 |
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| 作者单位： | 江南大学 生物工程学院, 江苏 无锡 214122,江南大学 生物工程学院, 江苏 无锡 214122,江南大学 生物工程学院, 江苏 无锡 214122,江南大学 生物工程学院, 江苏 无锡 214122,江南大学 生物工程学院, 江苏 无锡 214122;江南大学 食品科学与技术国家重点实验室, 江苏 无锡 214122,江南大学 生物工程学院, 江苏 无锡 214122;江南大学 食品科学与技术国家重点实验室, 江苏 无锡 214122 |
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| 摘 要： | 为了制备分子量分布集中的小分子透明质酸,在6g/L的高分子量透明质酸水溶液中加入一定梯度重组水蛭透明质酸酶,并控制水解时间。结果表明,通过控制加入重组水蛭HAase的量和水解时间,根据水解分子量下降规律,可以制备出分子量分布集中的小分子HA,包括水解终产物透明质酸四糖和六糖。同时,通过高效体积排阻色谱和琼脂糖凝胶电泳,可以证明水解产物的分子量分布集中。
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| 关 键 词： | 透明质酸 重组水蛭透明质酸酶 小分子透明质酸 高效体积排阻色谱 琼脂糖凝胶电泳 |
| 收稿时间： | 2015/4/3 0:00:00 |
Preparation of Small Molecular Hyaluronan by Enzymatic Hydrolysis in Vitro |
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| YUAN Panhong,KANG Zhen,JIN Peng,LIU Long,DU Guocheng and CHEN Jian.Preparation of Small Molecular Hyaluronan by Enzymatic Hydrolysis in Vitro[J].Journal of Beijing Technology and Business University:Natural Science Edition,2016,34(2):51-55, 82. |
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| Authors: | YUAN Panhong KANG Zhen JIN Peng LIU Long DU Guocheng and CHEN Jian |
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| Institution: | School of Biotechnology, Jiangnan University, Wuxi 214122, China,School of Biotechnology, Jiangnan University, Wuxi 214122, China,School of Biotechnology, Jiangnan University, Wuxi 214122, China,School of Biotechnology, Jiangnan University, Wuxi 214122, China,School of Biotechnology, Jiangnan University, Wuxi 214122, China;State Key Laboratory of Food Science and Technology of Ministry of Education, Jiangnan University, Wuxi 214122, China and School of Biotechnology, Jiangnan University, Wuxi 214122, China;State Key Laboratory of Food Science and Technology of Ministry of Education, Jiangnan University, Wuxi 214122, China |
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| Abstract: | In order to get concentrated distribution of small molecular hyaluronan, 6g/L of high molecular hyaluronic acid aqueous solution was prepared, added with a certain gradient of hyaluronidase, and controlling the hydrolysis time. The results showed that controlling the amount of hyaluronidase and hydrolysis time, particular molecular weight hyaluronic acid could be prepared according to the law of the hyaluronan molecular weight decreased,which included tetrasaccharide and hexasaccharide. Furthermore,through high performance size exclusion chromatography and agarose gel electrophoresis, we could prove that the molecular weight distribution of hydrolysate was concentrated. |
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| Keywords: | hyaluronan recombinant leech hyaluronidase small molecular hyaluronan high performance size exclusion chromatography agarose gel electrophoresis |
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