| 流式细胞仪法检测4HPR诱导Hela细胞凋亡的百分率 |
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| 引用本文： | 邵世和,李咏梅,陈曦,孙兰林,李薇. 流式细胞仪法检测4HPR诱导Hela细胞凋亡的百分率[J]. 北华大学学报(自然科学版), 1998, 0(2) |
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| 作者姓名： | 邵世和 李咏梅 陈曦 孙兰林 李薇 |
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| 作者单位： | 吉林医学院微生物教研室!吉林132001(邵世和，李咏梅，陈曦)，吉林医学院专科医院(孙兰林)，吉林医学院寄生虫教研室(李薇) |
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| 摘 要： | 目的：实验结果表明4HPR抑制Hela细胞生长的机理之一是诱导该细胞凋亡。本文用流式细胞仪检测不同浓度的4HPR作用不同时间Hela细胞凋亡的百分比及细胞周期变化。方法：体外细胞培养及流式细胞仪检测法。结果：2μg／m的4HPR作用24、48、72h后，Hela细胞的凋亡百分比分别为4.1％、6．6％、26％；4μg／ml的4HPR分别为5．1％、8．3％、28％、8μg／ml的4HPR分别为5．9％、6．2％、34％。细胞周期分布图中，G1细胞明显减少。结论：上述结果提示Hela细胞凋亡的百分比与4HPR的作用时间、作用浓度呈正相关。
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| 关 键 词： | 流式细胞仪 4HPR 细胞周期 细胞凋亡百分率 |
The percentage of 4HPR induced Hela cells apoptosis detected by flow cytometer |
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| Sbao Shihe, Sun Lanlin. The percentage of 4HPR induced Hela cells apoptosis detected by flow cytometer[J]. Journal of Beihua University(Natural Science), 1998, 0(2) |
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| Authors: | Sbao Shihe Sun Lanlin |
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| Abstract: | Objective:Experimental results showed that one of the mechanisms of 4HPR inhibit Hela cells is to induce apoptosis.Flow cytometer was used to detect the percentage and cell cycle changes of different concentration 4HPR acted on different stages Hela cells apoptosis.Method:Cell culture in vitro and flow cytome ter detectation.Results:After 24,48,72h acted by 2ug/ml 4HPR,the APO peroentage of hels celle were 4.1%,6.6%;by 4HPR were 5.1%,8.3%,28%; by 8ug/4ml 4HPR were 5.9%, 6.2%, 34%. In the distributions figure of cell cycle,G1 stage cells apparently reduced.Conclusion:The results showedthat the APO percentage of Hela cells was positive correlation with the time and concentration 4HPR action. |
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| Keywords: | Flow cytometer 4HPR Cell cycle Percentage of apoptosis |
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