珍珠岩-磷酸化聚乙烯醇生物材料的制备及其降解阿特拉津特性

 阿特拉津因其普遍性、高污染性和难降解性一直是历年来的研究热点.其降解方法很多,其中基于阿特拉津降解菌、生物固定化和一些无机材料的生物降解是最有效的.本文利用磷酸交联剂对聚乙烯醇(PVA)进行改性,以珍珠岩作为添加介质,对阿特拉津降解菌——Pseudomonas W4包埋固定,制备出一种新型珍珠岩-磷酸化聚乙烯醇(PPVA)生物活性材料(简称珍珠岩-PPVA),并对其最佳制备条件以及在不同反应条件下该材料降解阿特拉津的效果进行探讨.该材料最佳制备条件为,珍珠岩(粒径为0.60~2.00mm)/10% PVA(V/V)=35/65,Pseudomonas W4包埋量1.0g/L,磷酸盐浓度1.25mol/L;外加碳源、磷源能够促进其降解阿特拉津;珍珠岩-PPVA在pH值为5.99~9.03时具有较好的降解效果,其抗酸碱能力优于游离W4菌.说明珍珠岩-PPVA可有效去除阿特拉津,可以作为新型材料加以深入研究应用于废水或土壤中阿特拉津降解.

Preparation of Perlite-phosphorylated Polyvinyl Alcohol Based Biomaterial and Its Degradation Characteristics of Atrazine

Atrazine has become a research hot spot for many years due to its universality, high pollution, and difficult degradation. In the recent years, much research has been done on Atrazine. All sorts of degradation methods have been found. Among them, the bio-degradation is the most effective. Based on atrazine-degrading bacterium, immobilization method, and some materials, a new study is conducted. A kind of biologically active material, namely, perlite-phosphorylated polyvinyl alcohol (perlite-PPVA) is prepared with modified polyvinyl alcohol (PVA) by using phosphate, perlite, and atrazine-degrading bacterium: Pseudomonas W4. The effect of the material on the degradation of atrazine is discussed under the optimal preparation conditions and the other different reactive conditions.The results show that the best preparation conditions are perlite (particle sizeis 0.60-2.00mm)/10% PVA (V/V) =35/65, Pseudomonas W4 embedded volume is 1g/L, phosphate density is 1.25mol/L of perlite-PPVA. External carbon source and phosphorus source are able to promote its degradation ability of atrazine. The effect of perlite-PPVA on the atrazine degradation is better when the pH is in the range of 5.99-9.03, its ability of anti-acid is also better than the free bacterium. It shows that perlite-PPVA is feasible for the removal of atrazine, and could be used as a new material applied to the degradation of atrazine in waste water or soil after further research.