| 重组真菌细胞色素P450nor2在酵母细胞中的表达 |
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| 引用本文： | 陈舒丽,刘德立,郑永良,赵莉,张山,邹煜平.重组真菌细胞色素P450nor2在酵母细胞中的表达[J].华中师范大学学报(自然科学版),2006,40(2):252-255. |
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| 作者姓名： | 陈舒丽 刘德立 郑永良 赵莉 张山 邹煜平 |
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| 作者单位： | 华中师范大学,生命科学学院,武汉,430079 |
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| 摘 要： | 酿酒酵母（Saccharomyces cerevisiae）是一种理想的真核蛋白表达系统．将真菌细胞色素P450nor2基因亚克隆到酵母表达载体pAUR123中，构建重组表达质粒pAUR-P450nor2并转化酿酒酵母AH22，经Aureobasidin A筛选和菌落PCR鉴定得到阳性克隆．SDS-PAGE分析证实：重组的真菌细胞色素P450nor2在酵母细胞中实现了高表达．
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| 关 键 词： | 细胞色素P450nor2 酿酒酵母 克隆表达 菌落PCR |
| 文章编号： | 1000-1190（2006）02-0252-04 |
| 收稿时间： | 2005-09-10 |
| 修稿时间： | 2005-09-10 |
The expression of recombinant cytochrome P450nor2 in Saccharomyces cerevisiae |
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| CHEN Shu-li,LIU De-li,ZHENG Yong-liang,ZHAO Li,ZHANG Shan,ZOU Yu-ping.The expression of recombinant cytochrome P450nor2 in Saccharomyces cerevisiae[J].Journal of Central China Normal University(Natural Sciences),2006,40(2):252-255. |
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| Authors: | CHEN Shu-li LIU De-li ZHENG Yong-liang ZHAO Li ZHANG Shan ZOU Yu-ping |
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| Abstract: | Saccharomyces cerevisiae was a desired expression system for eukaryotic protein. Cytochrome P450nor gene was subcloned into the shuttle vector pAUR123 to yield the recombinant plasmid pAUR-P450nor2. The recombinant plasmid pAUR-P450nor2 was transformed into S. cerevisiae AH22 and got positive clone after the screening with Aureobasidin A and colony PCR. The SDS-PAGE showed that the expression product was recombinant cytochrome P450nor2 and got a high level. |
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| Keywords: | cytochrome P450nor2 Saccharomyces cerevisiae cloning and expression colony PCR |
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