赤霉素的固态分批补料培养技术及其分离提取工艺研究

经菌种筛选来串珠镰孢AS．3，2960菌株；对影响赤霉素产率的固态分批补料发酵的7个因素进行了正交试验，从而筛选出最佳发酵条件为：在32℃下，按培养基初始pH值4．5，接种量20％的比例，在第3天添加13．2％的玉米淀粉，从第3通气，共培养6d；对固 发批补料发酵与传统的液态发酵产生赤霉素能力进行了比较，结果表明：其固态分批补料培养技术为液态发酵的3－4．7倍；对发酵产物进行了分离提取效果的比较实验，结果表明：以10％的乙醇，在pH2．5下提取效果最佳；进行了多级逆流浸提试验，赤霉素含量达到0．70g/kgDMB（发酵物）。

Research on Gibberellin Production Prepared by Fed in Batches Solid-State Fermentation and Separated Distill Technique

This research decides Fusarium monilifome AS.3,2960 by screening. Six-level orthogonal tests were made with seven factors which influence on the capability of gibberellin; while the best ferment conditions were selected, which are in 32℃,the beginning pH 4.5 of culture medium ,20% of inoculating quantity, adding 13.2% corn starch in the third day, ventilating in the third day and the fermentation being 6 days. Under the same fermentation condition, the gibberellin production prepared by fed in batches solid-state and liquid-stat fermentation was compared. The results showed that the quantity of gibberellin production by fed in batches solid-state fermentation is about 3-4.3 times as much as that by liquid-state fermentation; with vary solvents and vary pH value the comparison tests of the effect of gibberellin separating-extraction showed that 10% alcohol was the best condition in pH 2.5 ; Throngh the multilevel backwash swimcarry test, the production rate of gibberellin reaches 0.70 g/kg DMB.