Zur Methodik der Artefaktausschaltung bei Anwendung von Diazoniumsalzen in der Histochemie (Phosphatasen,Esterasen usw.) |
| |
Authors: | W Meier-Ruge Elisabeth Meier |
| |
Institution: | (1) Abteilung für experimentelle Pathologie und Histochemie der Sandoz AG, Basel, Schweiz |
| |
Abstract: | Summary A post-incubation with 1% sulfanilic acid solution in 10% CH3COOH (approx. 2–10 min) or 5–10% potash alum or 0.5 mol AlCl3 in 0, 1n HCl-after the histochemical reaction with diazonium salt-prevents N2 formation. This gas originates from decomposition of tissue adsorbed diazonium salt which occurs after mounting in glycerin jelly. The inhibition of diazo-group disintegration depends, in the case of sulfanilic acid, on the formation of a diazoamino compound or double salt production with aluminium ions in the case of AlCl3. |
| |
Keywords: | |
本文献已被 SpringerLink 等数据库收录! |
|