Studies on the cytochemical localization of adenylate-cyclase activity inDugesia lugubris s.l. |
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| Authors: | Rita Pascolini Anna Maria Gargiulo A. Spreca A. Orlacchio |
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| Affiliation: | (1) Institutes of Normal Anatomy in the Faculties of Veterinary Medicine and of Biochemistry, Faculty of Pharmacy, University of Perugia, I-06100 Perugia, Italy;(2) Istituto di Chimica Biologica, Facoltà di Farmacia, via del Liceo, I-06100 Perugia, Italy |
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| Abstract: | Summary The localization of adenylate-cyclase activity inDugesia lugubris s.l. has been investigated cytochemically using 5 -adenylyl-imidodiphosphate as substrate. The enzyme was localized in mucous gland cells, in rhabdite cells, in intercellular spaces and also in nerve endings of this planarian. The presence of adenylate-cyclase on the membrane suggests that it might mediate different stimulus-secretion coupling by increasing cyclic AMP synthesis in specialized areas of the planarian.Supported in part by a grant from Consiglio Nazionale delle Ricerche, Rome.Acknowledgments. This work was performed with the technical assistence of the laboratory of Electron Microscopy of the University of Perugia. |
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