Cloning of a novel gene encoding human thioredoxin-like protein |
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Authors: | Yan Zhou Meihui Pan Jiangang Yuan Guangwei Du Boqin Qiang |
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Institution: | ZHOU Van, PAN Meihui, YUAN Jiangang, DU Guangwei and QIANG Boqin
State Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences , Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100005, China |
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Abstract: | mRNA differential display (DDRT-PCR) has been used to analyze different human fetal brain tissues of different developmental stages (13- and 33-week). According to the sequence of one EST obtained in this assay, a pair of primers have been designed to screen the arrayed human fetal brain cDNA library. A1 .2-kb cDNA clone has been found. This cDNA consists of an 867 bp open reading frame, a 132 bp 5' untranslated sequence and a 209 bp 3' untranslated sequence with a typical polyadenylation signal. The coding region predicts a protein of 289 amino acids. Its N-terminal of 105 residues is highly homologous to human thioredoxin, while no homology has been found in the databases with its C-terminal of 184 residues. Its N-terminal region also contains the conserved active site sequence CGPC (Cys-Gly-Pro-Cys) of thioredoxin. It was named human Thioredoxin-like gene (hTRXL). |
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Keywords: | DDRT-PCR thioredoxin fetal brain |
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